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          產品中心

          小鼠白介素1β(IL1b)微量上樣酶聯免疫吸附檢測試劑盒
          ELK1271MS
          規格: 價格:
          48T ¥1960.00
          96T ¥2800.00

          Overview 文獻

          Product name: Mouse IL1b(Interleukin 1 Beta) Microsample ELISA Kit
          Reactivity: Mouse
          Alternative Names: IL-1B;IL1-B; IL1-Beta; IL1F2; IL-1β; Interleukin-1 Family Member 2; Catabolin
          Assay Type: Sandwich
          Sensitivity: 6.4 pg/mL
          Standard: 1000 pg/mL
          Detection Range: 15.63-1000 pg/mL
          Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
          Assay Length: 3.5h
          Research Area: Cytokine;Tumor immunity;Infection immunity;
          Uniprot ID: P10749
          Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL1b in the samples is then determined by comparing the OD of the samples to the standard curve.

          標準曲線

          Concentration (pg/mL) OD Corrected OD
          1000.00 2.048
          500.00 1.541
          250.00 1.094
          125.00 0.845
          62.50 0.501
          31.25 0.269
          15.63 0.146
          0.00 0.000

          精密度

          Intra-assay Precision (Precision within an assay):CV%<8%

          Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

          Inter-assay Precision (Precision between assays):CV%<10%

          Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

          回收率

          Matrices listed below were spiked with certain level of recombinant IL1b and the recovery rates were calculated by comparing the measured value to the expected amount of IL1b in samples.
          Matrix Recovery range Average
          serum(n=5) %
          EDTA plasma(n=5) %
          Heparin plasma(n=5) %

          線性

          The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL1b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
          Matrix 1:2 1:4 1:8 1:16
          serum(n=5)
          EDTA plasma(n=5)
          Heparin plasma(n=5)

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