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          規格: 價格:
          48T ¥1680.00
          96T ¥2400.00

          Overview 文獻

          Product name: Human IL1b(Interleukin 1 Beta) ELISA Kit
          Reactivity: Human
          Alternative Names: IL-1B;IL1-B; IL1-Beta; IL1F2; IL-1β; Interleukin-1 Family Member 2; Catabolin
          Assay Type: Sandwich
          Sensitivity: 5.8 pg/mL
          Standard: 1000 pg/mL
          Detection Range: 15.63-1000 pg/mL
          Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
          Assay Length: 3.5h
          Research Area: Cytokine;Tumor immunity;Infection immunity;
          Uniprot ID: P01584
          Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL1b in the samples is then determined by comparing the OD of the samples to the standard curve.


          Concentration (pg/mL) OD Corrected OD
          1000.00 2.207 2.125
          500.00 1.613 1.531
          250.00 1.115 1.033
          125.00 0.757 0.675
          62.50 0.421 0.339
          31.25 0.297 0.215
          15.63 0.195 0.113
          0.00 0.082 0.000


          Intra-assay Precision (Precision within an assay):CV%<8%

          Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

          Inter-assay Precision (Precision between assays):CV%<10%

          Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.


          Matrices listed below were spiked with certain level of recombinant IL1b and the recovery rates were calculated by comparing the measured value to the expected amount of IL1b in samples.
          Matrix Recovery range Average
          serum(n=5) 92-105% 98%
          EDTA plasma(n=5) 83-95% 89%
          Heparin plasma(n=5) 79-93% 86%


          The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL1b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
          Matrix 1:2 1:4 1:8 1:16
          serum(n=5) 87-93% 91-103% 82-99% 99-104%
          EDTA plasma(n=5) 84-99% 81-95% 98-107% 96-101%
          Heparin plasma(n=5) 86-105% 84-98% 95-102% 88-106%


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