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          產品中心

          一步法人骨鈣素(OC)酶聯免疫吸附檢測試劑盒
          ELK009ES
          規格: 價格:
          48T ¥2240.00
          96T ¥3200.00

          Overview

          Product name: EasyStep Human OC(Osteocalcin) ELISA Kit
          Reactivity: Human
          Alternative Names: BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein
          Assay Type: Sandwich
          Sensitivity: 0.14 ng/mL
          Standard: 15 ng/mL
          Detection Range: 0.23-15 ng/mL
          Sample Type: Serum
          Assay Length: 1.5h
          Research Area: Metabolic pathway;Endocrinology;Hormone metabolism;Bone metabolism;
          Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OC, and the Human OC standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human OC. After TMB substrate solution is added, only those wells that contain Human OC and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OC in the samples is then determined by comparing the OD of the samples to the standard curve.

          標準曲線

          Concentration (ng/mL) OD Corrected OD
          15.00 2.126 2.025
          7.50 1.572 1.471
          3.75 1.116 1.015
          1.88 0.861 0.760
          0.94 0.538 0.437
          0.47 0.329 0.228
          0.24 0.192 0.091
          0.00 0.101 0.000

          精密度

          Intra-assay Precision (Precision within an assay):CV%<8%

          Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

          Inter-assay Precision (Precision between assays):CV%<10%

          Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

          回收率

          Matrices listed below were spiked with certain level of recombinant OC and the recovery rates were calculated by comparing the measured value to the expected amount of OC in samples.
          Matrix Recovery range Average
          serum(n=5) 87-99% 93%
          EDTA plasma(n=5) 87-99% 93%
          Heparin plasma(n=5) 81-95% 88%

          線性

          The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
          Matrix 1:2 1:4 1:8 1:16
          serum(n=5) 86-97% 88-95% 92-101% 87-98%
          EDTA plasma(n=5) 78-92% 82-96% 86-99% 93-101%
          Heparin plasma(n=5) 85-96% 86-98% 92-103% 87-93%

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