<track id="uzp5a"></track>

  • <track id="uzp5a"></track>
    <td id="uzp5a"><option id="uzp5a"></option></td>
    1. <p id="uzp5a"></p>

        1. <pre id="uzp5a"></pre>

          產品中心

          一步法人胱抑素C(Cys-C)酶聯免疫吸附檢測試劑盒
          ELK005ES
          規格: 價格:
          48T ¥2240.00
          96T ¥3200.00

          Overview

          Product name: EasyStep Human Cys-C(CystatinC) ELISA Kit
          Reactivity: Human
          Alternative Names: CST3; Cystatin 3; ARMD11; Post-Gamma-Globulin
          Assay Type: Sandwich
          Sensitivity: 25 pg/mL
          Standard: 5000 pg/mL
          Detection Range: 78.1-5000 pg/mL
          Sample Type: Serum, plasma
          Assay Length: 1.5h
          Research Area: Tags & Cell Markers;Cell Biology;Stem Cells;Cardiovascular
          Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Cys-C, and the Human Cys-C standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human Cys-C. After TMB substrate solution is added, only those wells that contain Human Cys-C and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.

          標準曲線

          Concentration (pg/mL) OD Corrected OD
          5000.00 2.781 2.716
          2500.00 1.701 1.637
          1250.00 0.906 0.842
          625.00 0.508 0.443
          312.50 0.319 0.254
          156.25 0.177 0.113
          78.13 0.120 0.056
          0.00 0.064 0.000

          精密度

          Intra-assay Precision (Precision within an assay):CV%<8%

          Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

          Inter-assay Precision (Precision between assays):CV%<10%

          Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

          回收率

          Matrices listed below were spiked with certain level of recombinant Cys-C and the recovery rates were calculated by comparing the measured value to the expected amount of Cys-C in samples.
          Matrix Recovery range Average
          serum(n=5) 88-99% 94%
          EDTA plasma(n=5) 102-110% 104%
          Heparin plasma(n=5) 98-104% 101%

          線性

          The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cys-C and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
          Matrix 1:2 1:4 1:8 1:16
          serum(n=5) 93-106% 88-102% 85-101% 96-107%
          EDTA plasma(n=5) 90-103% 94-108% 90-106% 98-105%
          Heparin plasma(n=5) 91-100% 98-107% 92-108% 93-102%

          產品成分表

          關閉

          在線咨詢

          Online consultation

          • 在線咨詢
          • 技術支持

          關注微信公眾號

          微信掃一掃立即咨詢

          微信掃一掃立即咨詢

          關閉
          亚洲熟女女同中文字幕|伊人久久大香线蕉综合色狠狠|中文字幕无码高av|精品无码毛片国产

          <track id="uzp5a"></track>

        2. <track id="uzp5a"></track>
          <td id="uzp5a"><option id="uzp5a"></option></td>
          1. <p id="uzp5a"></p>

              1. <pre id="uzp5a"></pre>